THUNDER Phospho-4EBP1 (T37/T46) TR-FRET Cell Signaling Assay Kit

Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-4EBP1 (T37/T46) in cell lysates.

Components: Eu-phospho-4EBP1 (250 µL), Acc-phospho-4EBP1 (1 mL), 4EBP1 control lysate (2 x 200 µL), 5X Lysis Buffer 2 (50 mL), 10X TR-FRET Detection buffer (2.5 mL), 100X Phosphatase Inhibitor Cocktail (2.5 mL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Phospho-4EBP1 (T37/T46).

Assay Target Class: Kinase.

SwissProt: Q13541.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).

• SKU: BIXKIT-4EBP1P-5000
• Manufacturer: BioAuxilium
• Manufacturer SKU: KIT-4EBP1P-5000
• Package Unit: 5000 Points
• Quantity Unit: PAK
• Reactivity: Human
• Application: Cell-based Assay
• Human Gene ID: 1978