THUNDER Phospho-BTK (Y223) TR-FRET Cell Signaling Assay Kit

Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-BTK (Y223) in cell lysates.

Components: Eu-phospho-BTK (5 µL), Acc-phospho-BTK (20 µL), BTK control lysate (100 µL), 5X Lysis Buffer #2 (1 mL), 10X TR-FRET Detection buffer (50 µL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Phospho-BTK (Y223).

Assay Target Class: Kinase.

SwissProt: Q06187.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).

• SKU: BIXKIT-BTKP-100
• Manufacturer: BioAuxilium
• Manufacturer SKU: KIT-BTKP-100
• Package Unit: 100 Points
• Quantity Unit: PAK
• Reactivity: Human
• Application: Cell-based Assay
• Human Gene ID: 695