THUNDER Total 4EBP1 TR-FRET Cell Signaling Assay Kit

Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Total 4EBP1 in cell lysates.

Components: Eu-Total AKT (250 µL), Acc-Total AKT (1 mL), AKT control lysate (2 x 200 µL), 5X Lysis Buffer 1 (50 mL), 10X TR-FRET Detection buffer (2.5 mL), 100X Phosphatase Inhibitor Cocktail (2.5 mL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Total 4EBP1.

Assay Target Class: Kinase.

SwissProt: Q13541.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).

• SKU: BIXKIT-4EBP1T-5000
• Manufacturer: BioAuxilium
• Manufacturer SKU: KIT-4EBP1T-5000
• Package Unit: 5000 Points
• Quantity Unit: PAK
• Reactivity: Human
• Application: Cell-based Assay
• Human Gene ID: 1978