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Encompassing Amino Acids: 26-143
Application: Useful as a positive control for Western blotting.
Description: Mouse T-cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as V-set and immunoglobulin domain-containing protein 9, VSIG9, V-set and transmembrane domain-containing protein 3, and VSTM3, GenBank Accession No. NM_001146325.1, a.a. 26-143 fused to Fc region of human IgG, expressed in a HEK293 cell expression system. MW = 42 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_001146325.1
Host Cell Line: HEK293
Purity: ≥90%
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: Fc fusion protein, N-terminal Avi-Tag, Biotin-Labeled
Biosafety Level: Not applicable (BSL-1)
References: 1. Yu, X., et al., Nat. Immunol. 2009; 10(1): 48-575.2. Stanietsky, N., et al., Proc. Natl. Acad. Sci. (USA). 2009; 106(42): 17858-17863.
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