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Encompassing Amino Acids: 2-325
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done according to TNIK Kinase Assay Kit (BPS Bioscience #78370) with various amounts of TNIK.
Description: Recombinant human TNIK (TRAF2 and NCK interacting kinase), encompassing amino acids, 2-325. This construct contains an N-Terminal GST-tag. A short peptide linker placed between GST and the protein includes a Thrombin Cleavage Site to facilitate removal of the GST portion. The recombinant protein was affinity purified.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT, and 2 mM glutathione
Genbank: NM_015028
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9UKE5
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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