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Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Background: TNIK (TRAF2 and NCK interacting kinase) is characterized by an N-terminal kinase domain and a C-terminal GCK domain (Germinal Center Kinase domain) that serves a regulatory function (1). TNIK is mainly expressed in the brain, heart, and spleen. It is a specific effector of RAP2 (RAP2A, member of RAS oncogene family), which regulates the actin cytoskeleton (2). TNIK autophosphorylation depends upon Lys54 in the ATP-binding pocket of its kinase domain and plays a major role in cytoskeleton regulation.
Contraindications: The final concentration of DMSO in the assay should not exceed 1%
Description: The TNIK Kinase Assay Kit is designed to measure TNIK kinase activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed
Supplied As: The assay kit comes in a convenient 96-well format, with enough purified recombinant TNIK kinase, kinase substrate, ATP and kinase assay buffer for 100 enzyme reactions.
Uniprot: Q9UKE5
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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