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Encompassing Amino Acids: 106-390
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using CSKtide substrate (0.2 mg/ml) and 10 µM ATP. Reaction was done at 30°C for 50 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)
Description: TNK1 (Tyrosine Kinase Non Receptor 1), also known as CD38 Negative Kinase 1 (GenBank Accession No. NM_003985), a.a. 106-390, with N-terminal GST-tag and Thrombin Cleavage Site, MW = 57 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 9mM Glutathione
Genbank: NM_003985
Storage Stability: At least 6 months at -80°C.
Tags: GST-Th-Tag
Uniprot: Q13470
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Felschow, D. (2000). BBRC. 273(1):294-301.2. Azoitei, N. et al. (2010). Oncogene. 26: 6536-6545.