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Application: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Contraindications: The TNKS1 (PARP5A) Chemiluminescent Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 10% DMSO solution and using 5 µl per well.
Description: The TNKS1 Chemiluminescent Assay Kit is designed to measure the activity of Tankyrase 1 (TNKS1, also known as PARP5A) for screening and profiling applications. TNKS1 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the TNKS1 (PARP5A) Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for TNKS1 reactions. First, histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the TNKS1 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.*NOTE: As of April 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The TNKS1 assay kit comes in a convenient 96-well or 384-well format, with purified TNKS1 enzyme, histone mixture, and PARP assay buffer for 100 or 400 enzyme reactions.
Uniprot: O95271
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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