Total Bile Acid (TBA) Colorimetric Assay Kit

Abbreviation: TBA

Target Synonym: TBA

Result Type: Quantitative

Detection Instrument: Microplate reader

Tested Sample Types: serum, animal tissue

Assay Time: 25 min

Sensitivity: 2.05 umol/L

Detection Range: 2.05-120 umol/L

Test Principle: With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.

• SKU: ELSE-BC-K181-M-500
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-E-BC-K181-M-500
• Green Labware: No
• Package Unit: 500 Assays
• Quantity Unit: PAK