THUNDER Total ERK1/2 TR-FRET Cell Signaling Assay Kit

Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Total ERK1/2 in cell lysates.

Components: Eu-Total ERK (5 µL), Acc-Total ERK (20 µL), ERK control lysate (100 µL), 5X Lysis Buffer 1 (1 mL), 10X TR-FRET Detection buffer (50 µL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Total ERK1/2.

Assay Target Class: Kinase.

SwissProt: P27361, P28482.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).

• SKU: BIXKIT-ERKT-100
• Manufacturer: BioAuxilium
• Manufacturer SKU: KIT-ERKT-100
• Package Unit: 100 Points
• Quantity Unit: PAK
• Reactivity: Human, Mouse (Murine)
• Application: Cell-based Assay
• Human Gene ID: 5595