Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
Background: Tryptophan 5-hydroxylase 1 and 2 (TPH1 and TPH2) are enzymes that catalyze the monooxygenation of tryptophan to 5-hydroxytryptophan (5-HTP) which is subsequently converted to serotonin. By catalyzing the rate-limiting step in the biosynthesis of serotonin, these enzymes also play key roles in the signaling of other neurotransmitters downstream of serotonin synthesis, like melatonin. Additionally, TPH enzymes may play a role in immune regulation through tryptophan depletion.
Description: The TPH1 Assay Kit is designed to measure TPH1 enzyme inhibition in a 384 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple - just mix two solutions, incubate, add quenching solution, and measure the fluorescence.
Storage Stability: At least 6 months from date of receipt when stored as directed.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Moran G. R., et al. J. Biol. Chem. 1998; 273:12259-12266.
2. Nowak E. C., et al., J. Exp. Med. 2012; 209:2127-2135.
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