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Encompassing Amino Acids: 151-466 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction wasperformed at room temperature in assaybuffer containing 50 mM MOPS, pH 7.0,60 µM tryptophan, 100 mM ammoniumsulfate, 100 µM ferrous ammonium sulfate,0.5 mM TCEP, 0.3 mM 6-methyltetrahydropterin, 0.05 mg/ml catalase, 1mM DTT and TPH2. Fluorescence signalwas measured at Em360nm/Ex300nm.
Description: Human tryptophanhydroxylase 2 (TPH2) also known asneuronal tryptophan hydroxylase,GenBank Accession No. NM_173353, a.a.151-466 (end) with an N-terminal His-tag,MW = 37 kDa, expressed in an E. coli cellexpression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04%Tween-20, 20% glycerol, and 160 mMimidazole
Genbank: NM_173353
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q8IWU9
Warnings: Avoidfreeze/thaw cycles. Storing dilutedenzyme is not recommended, ifnecessary, use carrier protein (BSA 0.1- 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Zill, P., et al., Mol. Psychiatr. 2004; 9: 1030-1036.
2. Sheehan, K., et al., Mol. Psychiatr.
2005; 10: 944-949.
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