Transporter™ 5 transfection reagent is the ready-to-use form of a proprietary linear polyethylenimine (PEI) derivative. Transporter™ 5 condenses DNA into positively charged complexes which readily enter the cell by endocytosis. The Transporter™ 5-DNA complex is highly resistant to endosomal degradation, allowing high transfection efficiency.
Transporter™ 5 transfection reagent effectively introduces DNA into HEK-293, CHO, COS, HeLa, insect (Sf9 and Sf21) and other eukaryotic cell lines. Transporter™ 5 is most compatible with plasmids up to 135,000 nucleotides in size, although larger plasmids can be used with some success.