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Encompassing Amino Acids: 442-796(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Activity determined using a DELFIA-assay with a poly-(Glu:Tyr 4:1)-biotin-coated plate, for 30 min. at room temperature.
Description: Human TRKA, also known as NTKR1 (Neurotrophic Tyrosine Kinase Receptor, type 1), GenBank Accession No. NM_002529, a.a. 442-796(end), with N-terminal GST-tag, MW=65 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 3 mM DTT, and 10% glycerol.
Genbank: NM_002529
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P04629
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Koch, A., et al. (2000). FEBS Lett. 469 (1): 72-62.
2. Wiesmann, C., et al. (1999). Nature 401 (6749): 184-83.
3. Yamashita, H. et al. (1999). J. Biol. Chem. 274 (21): 15059-65
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