Clone Name: SPM224
Light Chain: Kappa
Application Note: For IF: Use at an assay dependent dilution. For IHC-P: Use at a dilution of 1:50. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the researcher.
Positive Control: Skeletal or cardiac muscle
Form: Liquid
Buffer (with preservative): 10mM PBS pH7.4, 0.6% BSA, 0.09% Sodium azide.
Concentration: 0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Background: Tropomyosin is a dimeric coiled-coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F-actin filaments, and the lack of proline and tryptophan.
Antigen Species: Chicken
Immunogen: Full length native protein (purified) (Chicken).
Purification: Protein G purified
Conjugation: Unconjugated
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