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Applications: Monitor TRPC3 calcium channel activity
Screen for activators or inhibitors of TRPC3 calcium channel
Background: TRPC3 channel belongs to the transient receptor potential channel (TRP) superfamily which is divided into seven main subfamilies. TRP channels share a similar architecture of six-transmembrane domains and function as tetramers. Most channels are permeable to calcium and monovalent cations (Vassort et al., 2009). Specifically, TRPC3 channel belongs to a canonical TRP (TRPC) subfamily that is involved in calcium entry. TRPC3 is activated through phospholipase C-linked receptors. When expressed in HEK293 cells, TRPC3 behaves as a receptor activated channel with constitutive activity that cannot be further increased by store depletion (Trebak et al., 2002).
Biological Activity: N-terminal FLAG tagged human TRPC3 channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of TRPC3 was characterized by calcium assay. TRPC3 produces a constitutive Ca2+ entry in HEK293 cells. Addition of methacholine, an activator of phospholipase C-linked muscarinic receptors, significantly activates TRPC3.
Description: Stable, recombinant HEK293 cell line expressing human TRPC3 (transient receptor potential cation channel, subfamily C, member 3, accession number NM_003305).
Genbank: NM_003305
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains ~1 X 10^6 cells in 1 ml of 10% DMSO.
Uniprot: Q13507
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Albert A.P., Saleh S.N., and Large W.A. (2009). Current Medicinal Chemistry 16: 1158-1165.
2. Lievremont J., et al. (2004). Am J Physiol Cell Physiol 287: C1709-C1716.
3. Trebak M., et al. (2002). J. Biol. Chem. 277: 21617-21623.
4. Vassort G. and Alvarez J. (2009). Can. J. Physiol. Pharmacol. 87: 100-107.
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