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Applications: Monitor TRPC7 calcium channel activity
Screen for activators or inhibitors of TRPC7 calcium channel
Biological Activity: N-terminal FLAG tagged human TRPC7 channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The function of TRPC7 was characterized by calcium assay. In TRPC7-HEK293 cells, addition of methacholine, an activator of phospholipase C-linked muscarinic receptors, significantly activates TRPC7, resulting Ca2+ entry in the cells.
Description: Stable, recombinant HEK293 cell line expressing human TRPC7 (transient receptor potential cation channel, subfamily C, member 7, accession number NM_020389).
Genbank: NM_020389
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Store in liquid nitrogen immediately upon receipt.
Supplied As: Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO.
Uniprot: Q70T24
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Moran MM et al. (2011). Nat Rev Drug Discov. 10(8):601-620
2. Vassort G. and Alvarez J. (2009). Can. J. Physiol. Pharmacol. 87: 100-107
3. Lievremont J., et al. (2004). Am J Physiol Cell Physiol 287: C1709-C1716.
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