Species: Tobacco etch virus
Product Description: TurboTEVTM Protease contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), a cysteine protease that recognizes the cleavage site of Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser and cleaves between Gln and Gly/Ser. TurboTEV Protease is a restriction grade protease that has a robust activity at 4ºC with high specificity and great stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein. TurboTEV Protease is a 52 kDa protein with both GST and His tags so it can be removed by either Ni-chelating or Glutathione (GSH) resin.
Application Note: > 10 Units/μg. 1 Unit of TurboTEV Protease cleaves >85% of 3 μg of control substrate in 1 hour at 30ºC. (For detail information, please refer to the protocol.)
Form: Liquid
Buffer (with preservative): 25mM Tris-HCl (pH8.0), 50mM NaCl, 1mM TCEP, 50% Glycerol, no preservative.
Concentration: 2 mg/ml (Please refer to the vial label for the specific concentration.)
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ExpressionSystem: E. coli
Conjugation: Unconjugated
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