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Applications: Ideal to digest nucleic acids and to reduce viscosity during protein purification and sample preparation.
Assay Conditions: Reaction buffer of 50 mM Tris-HCl, pH 8.0 and 1 mM MgCl2.
Description: Ultra-pure recombinant form of Serratia marcescens extracellular endonuclease. Nonspecific endonuclease, hydrolyzes both single- and double-stranded nucleic acids (DNA and RNA) to 5'-phosphorylated oligonucleotides of 1-4 bases in length. MW = 27 kDa (homodimer). Expressed in E. coli. No detectable protease activity.TurboNuclease can be used to
reduce viscosity of cell lysates
reduce back pressure of column loading
remove nucleic acid contamination from sample preparations
reduce nucleic acid contamination of Ni column purification
reduce smearing in SDS-PAGE when used with 10%SDS or Gel Loading Dye
make whole cell lysates
reduce or prevent clumping of concentrated cells and thawed cells
replace crude DNase I in many applications
Endotoxin Level: Total endotoxin level is < 0.25 EU/1,000 units of TurboNuclease as determined by the LAL Gel-Clot Assay. No protease activity was detected.
Host Cell Line: E. coli
Purity: ≥99%
Storage Stability: >6 months at -20°C. Retains >80% activity after storage at RT for over 65 hours.
Supplied As: 250 units/µl in 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 5 mM MgCl2 and 50% glycerol
Unit Definition: One unit converts 1 OD260 of salmon sperm DNA into acid soluble nucleotides in 30 minutes at 37°C
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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