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Encompassing Amino Acids: 2-end
Applications: Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human UbcH5a (GenBank Accession No.NM_003338), a.a. 2-end, with N-terminal His-tag, MW=17 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 225 mMimidazole, 10% glycerol, and 3 mM DTT.
Genbank: NM_003338
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: P51668
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Markson G. et al., Genome Res. 19, 1905-1911 (2009).
2. Nuber U. and Scheffner M., J. Biol. Chem. 274 (11), 7576-7582 (1999).
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