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Encompassing Amino Acids: 2-end
Application: Useful in conjunction with E1 and E3 for the studies of enzyme kinetics, screening inhibitors and selectivity profiling.
Description: Human UbcH5b also known as UBE2D2 (GenBank Accession No. U39317.1), amino-acids 2-end, with N-terminal His tag, MW=18 kDa, expressed in an E.Coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween 20, 20% glycerol, 105 mM Imidazole
Genbank: U39317.1
Purity: ≥90%
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: N-terminal His-tag
Uniprot: P62837
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Markson G. et al., Genome Res. 19, 1905-1911 (2009).
2. Nuber U. and Scheffner M., J. Biol. Chem. 274 (11), 7576-7582 (1999).
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