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Encompassing Amino Acids: 1-154
Applications: Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 µl reaction mix(4 mM ATP, 5 mM MgCl2, 1 mM DTT,UBE1 (0.5 µg), UbcH7, Biotin-Ubiquitin(0.5 µg) and Smurf2 (1.5 µg) in 50 mMTris, pH 7.4) for 30 min at 37°C. Add toStreptavidin-coated plates for 30 minat 37°C. Incubate with anti-Smurf2, thenHRP-labeled secondary antibody, andchemiluminescent detection.
Description: UbcH7, also known as UBE2L3 (GenBank Accession No. NM_003347), a.a. 1-154 (end), with C-terminal His-tag, MW = 18.7 kDa, expressed Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 0.05% Tween-20, 50% glycerol and 1 mM DTT
Genbank: NM_003347
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P68036
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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