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Encompassing Amino Acids: 2-1058(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done according to UBE1 Inhibitor Screening Assay Kit (BPS Bioscience #79957) with UBE1 titrated from 6000 ng /rxn to 0 ng/rxn.
Description: Human Ubiquitin Activating Enzyme (E1) (UBE1), also known as UBA1, GenBank Accession No. NM_003334, a.a. 2-1058(end) with N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW = 145 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 20% Glycerol and Glutathione.
Genbank: NM_003334
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal GST-tag
Uniprot: P22314
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ramser, J., et al., AJHG. 2018;82(1): 188-193.
2. Leidecker, O., et al., Cell Cycle. 2012; 11(6): 1142-1150.
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