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Applications: Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done according to UBE1 Inhibitor Screening Assay Kit (BPS Bioscience #79957) with UBE1 titrated from 1500 ng/rxn to 0 ng/rxn.
Description: Human UBE1 (GenBank Accession No. NM_003334), a.a. 2-end, with N-terminal FLAG-tag, MW=118 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol
Genbank: NM_003334
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: P22314
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Su Z.L. et al., Leuk. Lymphoma 49 (9), 1821-1822 (2008).
2. Ramser J. et al., Am. J. Hum. Genet. 82 (1), 188-193 (2008).
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