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Applications: Useful for the study of ubiquitin enzyme kinetics, and as a positive control for Western blot studies.
Background: UBE2D2(UBC4) Protein or ubiquitin-conjugating enzyme E2D2 is a member of the E2 ubiquitin-conjugating enzyme family which functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. UBE2D2 specifically ubiquitinates E6AP and in vivo inhibition of UBE2D2 can lead to inhibition of E6-stimulated p53 degradation (1). UBE2D2 could conjugate ubiquitin to target proteins in an E6AP-dependent manner.
Description: Recombinant full-length Xenopus UBE2D2, also known as UBC4, PUBC1, UBC4/5, UBCH5B, and E2(17)KB2. Genbank Accession No. NM_001093036, with an N-terminal His-tag, expressed in E. coli. MW=17 kDa.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, and 25% glycerol.
Genbank: NM_001093036
Storage Stability: At least 6 months at -70⁰C. Avoid freeze/thaw cycles.
Tags: N-Terminal His-Tag
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Mondal, S., et al., Anal. Biochem. 2016; 510: 41-51.2. Rolfe, M., et al., Proc. Nat. Acad. Sci. 1995; 92: 3264-3268.
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