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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: UBE2E3 also known as UBCH9 or ubiquitin-conjugating enzyme E2E 3 is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination, the covalent attachment of the protein ubiquitin (Ub) to other cellular proteins is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation that involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s(1). UBE2E3 is highly expressed in skeletal muscle (2). UBE2E3 is essential for the proliferation of RPE-1 cells and is downregulated during RPE layer maturation in the developing mouse eye. These findings indicate that UBE2E3 is a major enzyme in modulating the balance between RPE cell proliferation and differentiation.
Description: Recombinant full length human UBE2E3, also known as UBCH9 and UbcM2. Genbank Accession NM_006357, with an N-terminal His-tag, expressed in E. coli cells. MW = 25kDa.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Genbank: NM_006357
Storage Stability: At least 6 months at -80⁰C. Avoid freeze/thaw cycles.
Tags: N-terminal His-tag
Uniprot: Q969T4
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Matuschewski, K., et.al. J. Biol. Chem. 271: 2789-2794, 1996.2. Ito, K., et.al. Cytogenet. Cell Genet. 84: 99-104, 1999.
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