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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: UBE2G1 (UBC7) or ubiquitin-conjugating enzyme E2G 1 is a member of the E2 ubiquitin-conjugating enzyme family and catalyzes the covalent attachment of ubiquitin to other proteins which are involved in degradation of muscle-specific proteins. UBC7 is highly expressed in skeletal muscle (1). UBC7 is involved in the selective ubiquitylation of different substrates by the same E3 complex (2).
Description: Recombinant full length Human UBE2G1, also known as UBC7, E217K, and UBE2G. Genbank Accession NM_003342, N-terminal His-tag, expressed in E. coli cells. MW = 21kDa.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Genbank: NM_0033
Storage Stability: At least 6 months at -80⁰C. Avoid freeze/thaw cycles.
Tags: N-terminal His-tag
Uniprot: P62253
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Watanabe, T.K., et.al., Cytogenet. Cell Genet. 1996; 74: 146-148.2. Shibata, E., et al., Mol Cvell Biol. 2011 Aug.
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