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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: UBE2K also known as UBC1 or ubiquitin-conjugating enzyme E2K belongs to the ubiquitin-conjugating enzyme family which interacts with RING finger proteins. UBE2K can ubiquitinate huntingtin, the gene product for Huntington's disease. Huntingtin is ubiquitinated in peripheral cells, suggesting a role for UBE2K in the degradation of huntingtin (1). UBE2K plays a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases (2). UBE2K also plays a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation.
Description: Recombinant full length UBE2K (UBC1) protein also known as E2-25K, HIP2, HYPG, LIG, and UB1. Genbank Accession NM_005339, with an N-terminal His-tag, expressed in E. coli cells. MW = 25kDa.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Genbank: NM_005339
Storage Stability: At least 6 months at -80⁰C. Avoid freeze/thaw cycles.
Tags: N-terminal His-tag
Uniprot: P61086
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kalchman, M. A. et.al. J. Biol. Chem. 271: 19385-19394, 1996. 2. De Pril, R. et al. Mol Cell Neurosci. 2007 Jan;34(1):10-9.
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