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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: UBE2O or ubiquitin-conjugating enzyme E2O is a part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin. HIV-1 Tat has been identified to have a physical interaction with ubiquitin-conjugating enzyme E2O (UBE2O) in human HEK293 and Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses (1).
Description: Recombinant full-length human UBE2O, also known as E2-230K, FLJ12878, and KIAA1734. Genbank Accession BC022237 with an N-terminal GST tag, expressed in Sf9 cells via a baculovirus expression system. MW = 90kDa.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Genbank: BC022237
Storage Stability: At least 6 months at -80⁰C. Avoid freeze/thaw cycles.
Tags: N-terminal GST-Tag
Uniprot: Q9C0C9
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Rumbaugh JA. et.al. Neurobiol Dis, 2012 Aug 25.
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