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Encompassing Amino Acids: 2-223(end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and UCHL1. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation of 340 nm, and emission at 460 nm.
Description: Human Ubiquitin Carboxyterminal Hydrolase (UCHL1), also known as PGP9.5, GenBank Accession No. NM_004181, a.a. 2-223, with N-terminal His-tag, expressed in E. coli. expression system. MW=26 kDa.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 10% Glycerol and Imidazole.
Genbank: NM_004181
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal His-tag
Uniprot: P09936
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
References: 1. Gong, B., et al., (2006). Cell 126 (4): 775- 788.
2. Healy DG, et al., (2005). Cell Tissue Res. 318 (1): 189-94.
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