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Encompassing Amino Acids: 2-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
Description: Human ULK1 (UNC-51-Like Kinase-1), also known as ATG1 (Autophagy related protein 1), GenBank Accession No. NM_003565, a.a. 2-end with N-terminal FLAG-tag, MW = 113 kDa, expressed in FreeStyle 293-F cells.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 100 µg/ml FLAG peptide, 20% glycerol and 3 mM DTT.
Genbank: NM_003565
Host Cell Line: FreeStyle 293-F cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: O75385
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ganley I.G. et al., J. Biol. Chem. 284 (18), 12297-12305 (2009).
2. Chan E.Y. et al., Mol. Cell. Biol. 29 (1), 157-171 (2009).
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