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Application: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and High Throughput (HTS) applications.
Background: ULK1 (unc-51 like autophagy activating kinase 1) is a serine/threonine protein kinase that plays a critical role during the initial stages of autophagy in response to nutrient starvation. The conserved C-terminal domain of ULK1 controls the function and the localization of the protein. Knockdown of ULK1 inhibits the autophagic response and prevents rapamycin-induced autophagy, consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy (1). Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of the ULK1 protein (2). ULK1 is phosphorylated upon activation of the mTOR pathway in a nutrient starvation-regulated manner. Alterations in ULK signaling pathways may be involved in the formation of autophagy-regulated Lewi bodies, which have been associated with Parkinson's disease (3).
Contraindications: The final concentration of DMSO in the assay should not exceed 1%.
Description: The ULK1 Kinase Assay Kit is designed to measure ULK1 activity for screening and profiling applications, using Kinase-Glo® MAX as a detection reagent.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The ULK1 Assay Kit comes in a convenient 96-well format, with enough purified ULK1, MBP substrate, ATP, and kinase assay buffer for 96 enzyme reactions.
Uniprot: O75385
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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