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Application: Negative control for lentivirus-transduced PRAME TCR-T cells
Background: PRAME (Preferentially Expressed Antigen in Melanoma) is a protein with a profile of expression in normal tissues
highly restricted to testis, ovarium, and endometrium. However, it is found at high levels in several cancer types,
such as melanoma, breast, and lung cancer. It is also found in cells of patients with AML (acute myeloid leukemia)
and Hodgkin's lymphoma. Overexpression seems to block retinoic acid-mediated cell proliferation, differentiation,
and apoptosis, contributing to tumorigenesis. Its expression pattern makes it an attractive target for
immunotherapy. It is a membrane-bound protein, and it is thus a good target for TCR (T cell receptor)-T cells and
anti-PRAME vaccines. Several clinical trials are ongoing and have demonstrated the clinical potential of targeting
PRAME in melanoma, lung cancer and other solid tumors. Further studies into the functions of this protein will
bring new clinical advances in cancer therapy.
Description: The Untransduced T Cells (PRAME TCR-T Negative Control) were produced by mock lentiviral transduction of human primary CD4+ and CD8+ T cells. These cells are subjected to comparable manipulations as TCR (T cell receptor)-T cells: activation, spinoculation (without lentivirus), and antigen specific stimulation. These T cells are designed as a negative control in experiments using lentivirus-transduced TCR-T cells, such as PRAME TCR-T Cells (#82391).
Mycoplasma Testing: The cells have been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Uniprot: P78395
Warnings: Avoid freeze/thaw cycles.Donors have been screened and determined negative for:Hepatitis B (anti-HBc EIA, HBsAg EIA)Hepatitis C (anti-HCV EIA)Human Immunodeficiency Virus (HIV-1/HIV-2 plus O)Human T-Lymphotropic Virus (HTLV-I/II)HIV-1/HCV/HBVWest Nile VirusTrypanasoma cruziNote: Testing cannot guarantee that any sample is completely virus-free. These cells should be treated as potentially infectious and appropriate biological safety level 2 (BSL-2) precautions should be used.
Biosafety Level: BSL-1
References: Kropp K.N., et al., 2020 PLOS One 15(9): e0238875.Salmaninejad A., et al., 2016 Immunol Invest 45(7):619-40.
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