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Encompassing Amino Acids: 2-798(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP10 in a 50 µL volume. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.
Description: Human USP10 (Ubiquitin-Specific Protease 10) or Ubiquitin C-terminal Hydrolase 10, GenBank Accession No. NM_005153, a.a. 2-798(end) with an N-terminal FLAG-tag, MW=88 kDa, expressed in a baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 10% glycerol, and 3 mM DTT.
Genbank: NM_005153
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: Q14694
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Faus, H., et al. (2005). Mol. Cell. Endocrinol. 245(1-2):138-46.
2. Soncini, C., et al. (2001). Oncogene 20(29):3869-79.
3. Yuan, J., et al. (2010). Cell 140(3): 384-96.Application Reference: Oi, N., et al. Oncogene. 2015 May 14;34(20):2660-71. Resveratrol induces apoptosis by directly targeting Ras-GTPase-activating protein SH3 domain-binding protein 1
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