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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: USP13 was incubated in 50 mM Tris-HCl, 0.5 mM EDTA, 0.05% Tween 20, 1 mM DTT with 500 nM Ubiquitin-AMC. The reaction was incubated at room temperature for 30 minutes. Fluorescence intensity was measured at λexc/λem of 360nm/460nm using a Tecan M1000 plate reader.
Background: USP13 (ubiquitin carboxyl-terminal hydrolase 13), also known as isopeptidase T 3 (ISOT-3) is a DUB (deubiquitination) enzyme. It participates in multiple crucial cellular processes, such as autophagy, DNA damage response, ERAD (endoplasmic reticulum-associated degradation) by deubiquitinating proteins involved in those functions. It also plays roles in diseases such as cancer and neurodegeneration. It can play both a suppressor and activator role in tumorigenesis. For instance, low levels of USP13 correlate with low levels of PTEN (phosphatase and tensin homolog), as UPS13 stabilizes the protein. A deeper understanding of all its cellular roles and how to best target its function in a specific context may prove beneficial in cancer therapy.
Description: Recombinant human USP13 (ubiquitin carboxyl-terminal hydrolase 13), encompassing amino acids 2-863(end). This construct contains an N-terminal His-tag (6xHis) and a
C-terminal FLAG-tag. This protein was affinity purified.
Format: Aqueous buffer solution.
Formulation: 45 mM Tris-HCl, pH 8.0, 300 mM NaCl, 2.48 mM KCl, 0.045% Tween-20, 10% glycerol, 100 µg/ml FLAG peptide
Genbank: NM_003940.3
Purity: ≥90%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-Tag, C-terminal FLAG-Tag
Uniprot: Q92995
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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