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Encompassing Amino Acids: 2-494(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP14 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.
Description: Human USP14 (Ubiquitin-Specific Protease 14) or TGT (tRNA-guanine transglycosylase), GenBank Accession No. NM_005151, a.a. 2-494(end) with an N-terminal FLAG-tag, MW=57 kDa, expressed in a baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 10% glycerol, and 3 mM DTT.
Genbank: NM_005151
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: P54578
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Chen, P.C., et al. (2009). J. Neurosci. 29(35):10909-19.
2. Chen, Y.C., et al. (2010). RNA 16(5):958-68.
3. Nagai, A., et al. (2009). Biochem. Biophys. Res. Commun. 379(4):995-1000.
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