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Applications: This enzyme is active in the Ub-CHOP assay.
Assay Conditions: The activity of USP15 and USP2core are measured with 100 nM DiUb48 in 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM DTT, 0.02% Tween-20
Background: USP15 is a deubiquitylase that associates with the COP9 signalosome (CSN) complex. It is able to regulate the NF-κappaB pathway by deubquitylating IkappaBalpha2.
Description: Human USP15 recombinant protein, with N-terminal SUMO- and His-tags, expressed in E. coli.
Format: Aqueous buffer solution
Formulation: 50 mM Na phosphate pH 7.5, 50 mM NaCl, 1 mM DTT
Storage Stability: Store at or below -80°C. Stable as supplied for up to 1 year when stored dessicated at -80°C.
Tags: N-terminal SUMO-His-tags
Uniprot: Q9Y4E8
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Schweitzer K., et al., CSN controls NF-κB by deubiquitinylation of IκBα. EMBO J., 26(6):1532-41 (2007).
2. Hetfeld, BK., et al., The zinc finger of the CSN-associated deubiquitinating enzyme USP15 is essential to rescue the E3 ligase Rbx1. Curr Biol, 15(13):1217-21 (2005).
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