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Applications: This enzyme is active in the Ub-CHOP assay.
Assay Conditions: Assay Conditions: 50 nM USP51, 200 nM substrate, 50 mM HEPES, pH 7.5, 150 mM NaCl, 5 mM DTT, 0.05% CHAPS
Background: USP51, a member of the USP family of cysteine proteases, contains a UBP-type Zn-finger and is expressed in the prostate, brain lung, aorta, and kidney.
Description: Human USP51 recombinant protein (Genbank Accession No. Q70EK9), with N-terminal SUMO- and His-tags, expressed in E. coli.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM DTT, 10% glycerol
Genbank: Q70EK9
Storage Stability: Store at or below -80°C. Stable as supplied for up to 1 year when stored dessicated at -80°C.
Tags: N-terminal SUMO-His-tags
Uniprot: Q70EK9
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Quesada, V., et al., Cloning and enzymatic analysis of 22 novel human ubiquitin-specific proteases. Biochem Biophys Res Commun. 314:54-62 (2004)
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