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Description: Recombinant humanvascular endothelial growth factor(VEGF)165 expressed in CHO cells.Human VEGF exists in multiplemolecular variants and are namedbased on the number of amino acidsthey contain. VEGF165 is the mostcommon form of VEGF.
Endotoxin Level: Endotoxin level wasfound to be < 0.1 ng/g (1EU/g), usingthe LAL gel clot method.
Format: lyophilized protein
Formulation: Recombinant VEGFlyophilized from 0.2 µm filtered 20 mMPB solution, pH 7.0.
Genbank: NP_001165097
Host Cell Line: CHO
Purity: >95%, as determined by SDSPAGEand HPLC.
Reconstitution: Briefly centrifuge the vial, followed by reconstitution in distilled water to a concentration of ≥0.1 mg/ml. This solution can then be further diluted into other buffers containing carrier protein (0.1% BSA).
Storage Stability: The lyophilized protein isstable for at least 2 years from date ofreceipt when stored at -20°C. Uponreconstitution, store in working aliquotsat 2 - 8° C for up to one month, or at-20°C for up to six months, in thepresence of a carrier protein. Avoidrepeated freeze/thaw cycles.
Uniprot: P15692-4
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Reproduction. 2009; 138: 667-677.
2. Ann. Onc. 2009; 20: 1639-1646.
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