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Encompassing Amino Acids: 805-1356
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in Kinase buffer containing 0.2 mM DTT using Poly- (Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Description: Human VEGFR2 (GenBank Accession No. AF035121), a.a. 805-1356 with N-terminal GST-tag, MW=87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween, 20% glycerol, 3 mM DTT
Genbank: AF035121
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P35968
Unit Definition: One unit of KDR activity is defined as the amount of enzyme required to produce 1 pmol of phosphorylated Tyr peptide 1 per min at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Meyer, M. et al., EMBO J. 18, 363-374 (1999).
2. Rahimi, N. et al., J. Biol Chem 275, 16986- 16992 (2000).
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