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Encompassing Amino Acids: 2-334(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human WDR5 (WD Repeat Domain 5),also known as SWD3 or BIG-3,GenBank Accession No. NM_017588,a.a. 2-334(end), with N-terminal FLAGtag,MW=37 kDa, expressed in abaculovirus-infected Sf9 insectexpression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 µg/ml FLAG peptide
Genbank: NM_017588
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-tag
Uniprot: P61964
Warnings: Avoidfreeze/thaw cycles. Storing diluted enzymeis not recommended.
Biosafety Level: Not applicable (BSL-1)
References: 1. Trievel, R.C., Shilatifard, A. Nature Struct. Mol. Biol. (2009). 16 (7): 678-80.
2. Wang, Y.Y., et al. Proc. Natl. Acad. Sci. (2010). USA 107(2):815-20.
3. Odho, Z., et al. J. Biol. Chem. (2010) .285(43):32967-76.