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Encompassing Amino Acids: 2-334 (end)
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human WDR5 (WD Repeat Domain 5), also known as SWD3 or BIG-3, GenBank Accession No. NM_017588, a.a. 2-334(end), with N-terminal Avi-His-tag, MW=39 kDa, expressed in Sf9 insect cells via a baculovirus expression system. This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation is confirmed to be ≥90%.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol.
Genbank: NM_017588
Host Cell Line: Sf9 cells
Purity: ≥90%
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1-0.5%).
Tags: Avi-His-tag
Uniprot: P61964
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Trievel, R.C., Shilatifard, A. (2009). Nature Struct. Mol. Biol. 16 (7): 678-80.2. Wang, Y.Y., et al. (2010). Proc. Natl. Acad. Sci. USA 107(2):815-20.3. Odho, Z., et al. (2010). J. Biol. Chem. 285(43):32967-76.
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