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Encompassing Amino Acids: 215-646 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using Poly-(Lys4:Tyr) substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min.Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)
Description: Human Wee1 protein, also known as Wee1A Kinase, GenBank Accession No. NM_003390, amino acids 215-646 (end), with an N-terminal GST-Thrombin tag, expressed in a Baculovirus infected Sf9 cell expression system. MW = 75 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 2mM glutathione
Genbank: NM_003390
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: N-terminal GST-Thrombin-Tag
Uniprot: P30291
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Parker, L., et al., Science. 1992; 257(5078):1955.2.Lundgreen, K, et al., Cell. 1991 Mar; 64(6):1111-1122.
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