Product Description: Wnt3a Surrogate protein is a Wnt recombination protein that plays an important role in embryonic development, adult tissue homeostasis and injury repair. Wnt3a Surrogate protein, consisting of the domain and Fc portion of Wnt3a, helps organoids expand and maintain a source of Wnt. Organoids are a rapidly evolving model system for tissue development, and Wnt3a is considered as the essential protein for organoid growth. Wnt3a or the small molecule glycogen synthase kinase 3 (GSK3) inhibitor CHIR are commonly used as Wnt sources in experiments. However, the application of Wnt3a is hampered by Wnt lipidation, and cross-reactivity with Wnt frizzled (Fzd). Therefore, Wnt3a Surrogate protein was used instead of Wnt3a, as a water-soluble and Fzd-specific alternative Wnt agonist. And the Wnt3a Surrogate protein exhibits tissue selectivity consistent with Fzd isoform specificity[1][2][3]. Wnt3a Surrogate Protein, Human (HEK293, Fc) is a recombinant Wnt3a protein of human origin. It is expressed by HEK293 cells with an Fc tag.
Formula: /(Gene_ID)/(Accession)
References: [1]Janda CY, et al. Surrogate Wnt agonists that phenocopy canonical Wnt and β-catenin signalling. Nature. 2017 May 11;545(7653):234-237. /[2]Chen H, et al. Development of Potent, Selective Surrogate WNT Molecules and Their Application in Defining Frizzled Requirements. Cell Chem Biol. 2020 May 21;27(5):598-609.e4. /[3]Miao Y, et al. Next-Generation Surrogate Wnts Support Organoid Growth and Deconvolute Frizzled Pleiotropy In Vivo. Cell Stem Cell. 2020 Nov 5;27(5):840-851.e6. /[4]He S, et al. Wnt3a: functions and implications in cancer. Chin J Cancer. 2015 Sep 14;34(12):554-62. /[5]Thomas BL, et al. WNT3A-loaded exosomes enable cartilage repair. J Extracell Vesicles. 2021 May;10(7):e12088.
Molecular Weight: 66-72 kDa.
Compound Purity: 98.0
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