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Encompassing Amino Acids: 120-356
Applications: Useful in binding and inhibition studies of Caspase 9, screening inhibitors, and selectivity profiling.
Assay Conditions: 50 mM Tris-HCl, pH 7.4, 25 mM NaCl, 0.05% Tween-20, 0.01% BSA, 0.5 nM FAM-SMAC peptide. Incubate 30 minutes at room temperature. Fluorescence polarization is measured at exc 475 nm, em 528 nm.
Description: Human x-linked inhibitor of apoptosis (XIAP), also known as IAP-like protein, API3, X-linked IAP, and BIRC4, a.a. 120 - 356 containing Bir2 and Bir3 domains (GenBank Accession No. NM_001167) with N-terminal His-tag, MW = 28.3 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.
Genbank: NM_001167
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P98170
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Nikolovska-Coleska, Z., et al., Anal Biochem. 2008 Mar 1;374(1):87-98.
2. Verhagen, A.M., et al., Cell Death Differ. 2007 Feb;14(2):348-57
3. Fulda, S., Vucic., D., Nat Rev Drug Discov. 2012 Feb 1;11(2):109-24
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