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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done inKinase buffer containing 0.2 mM DTTusing Poly-(Glu4:Tyr) substrate (0.2mg/ml) and 20 µM ATP. Reaction wasdone at 30°C for 45 min. Amount of ATPtransferred was calculated using Kinase-Glo reagent (Promega).
Description: Human ZAP70, alsoknown as ZAP-70, zeta-chain associatedprotein kinase, syk-related tyrosinekinase, SRK, or STD, GenBankAccession No. NM001079, full lengthwith N-terminal GST tag, MW = 96 kDa,expressed in a Baculovirus infected Sf9cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCL, pH 8.0,110 mM NaCl, 2.2 mM KCl, 3 mM DTT,20% glycerol and 1.6 mM glutathione
Genbank: NM_001079
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P43403
Warnings: Avoidfreeze/thaw cycles. Storing diluted protein isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Ishaq, M., et al. J Immunol. 2007 Sep 15;179(6):3851-8.
2. Siggs, O., et al. Immunity. 2007 Dec;27(6):912-26.
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