ZymoBIOMICS Spike-in Control II (Low Microbial Load) (500 µl x 1)

Whole cell spike-in control with log abundance distribution for limit detection and absolute cell quantitification of microbial samples.

Description: ZymoBIOMICS Spike-in Control II (Low Microbial Load) consists of three bacteria strains, Truepera radiovictrix, Imtechella halotolerans and Allobacillus halotolerans. When spiked into a microbial sample, this product will serve as an in situ positive control for DNA-sequencing-based microbiome measurements. Of the three chosen bacteria, Imtechella halotolerans is Gram-negative and Allobacillus halotolerans is Gram-positive. Truepera radiovictrix is resistant to lysozyme lysis and has very high GC content. They represent different challenges in NGS-based analysis. Moreover, with accurately quantified cell number and a log abundance distribution, this standard enables absolute cell number quantification in cases such as pathogen load detection.

• Absolute Quantification: Enables cell number measurements from low bacterial load samples using Next-Gen Sequencing.
• In situ Quality Control: Ensures each sample is quantified accurately.
• Log Abundance Distribution: Three microbes alien to the human microbiome in log-distribution from 103 to 105 cells.

• SKU: ZYMD6321
• Manufacturer: Zymo Research
• Manufacturer SKU: D6321
• Package Unit: 25 Preps
• Quantity Unit: STK